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important industrial production backbone  (Addgene inc)


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    Addgene inc important industrial production backbone
    Important Industrial Production Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet28+gfp+plasmid/pET28%3AGFP+(Plasmid+%2360733)/bio_rxiv__2025__07__05__663304-174-9-18
    Average 93 stars, based on 14 article reviews
    important industrial production backbone - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid , which was a gift from Mathew Bennett (Addgene plasmid # 60733; http://n2t.net/addgene :60733; RRID: Addgene_60733 ). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD-mTagBFP2 plasmid , a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene :34632; RRID: Addgene_34632 ).

    Article Title: Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid (Shis and Bennett, 2013), which was a gift from Mathew Bennett (Addgene plasmid # 60733; http://n2t.net/ addgene: 60733; RRID:Addgene_60733). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD- mTagBFP2 plasmid (Subach et al., 2011), a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene: 34632; RRID:Addgene_34632).

    Article Title: A Fitness–Entropy Compensation effect set the trade-off between growth and gene expression in cell populations
    Article Snippet: .. The E. coli BL21(DE3) strain, one of the most important industrial production backbone, carrying the pET28:GFP plasmid ( Addgene #60733 ) was used to investigate the burdensome T7 expression system. ..

    Article Title: Tight Junction Modulatory Fusion Peptide (ADT-6) Enhances GFP Protein Permeability through the Paracellular Pathway in Caco-2 Cell Lines: An In-Vitro Study
    Article Snippet: .. The pET28-GFP plasmid (Addgene plasmid #60733) was employed to amplify GFP. ..

    Article Title: DNA/polysome phase separation and cell width confinement couple nucleoid segregation to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid , which was a gift from Mathew Bennett (Addgene plasmid # 60733 ; http://n2t.net/addgene:60733 ; RRID:Addgene_60733). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD-mTagBFP2 plasmid , a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene:34632 ; RRID:Addgene_34632).

    Variant Assay:

    Article Title: Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid , which was a gift from Mathew Bennett (Addgene plasmid # 60733; http://n2t.net/addgene :60733; RRID: Addgene_60733 ). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD-mTagBFP2 plasmid , a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene :34632; RRID: Addgene_34632 ).

    Article Title: Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid (Shis and Bennett, 2013), which was a gift from Mathew Bennett (Addgene plasmid # 60733; http://n2t.net/ addgene: 60733; RRID:Addgene_60733). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD- mTagBFP2 plasmid (Subach et al., 2011), a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene: 34632; RRID:Addgene_34632).

    Article Title: DNA/polysome phase separation and cell width confinement couple nucleoid segregation to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid , which was a gift from Mathew Bennett (Addgene plasmid # 60733 ; http://n2t.net/addgene:60733 ; RRID:Addgene_60733). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD-mTagBFP2 plasmid , a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene:34632 ; RRID:Addgene_34632).

    Derivative Assay:

    Article Title: Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid , which was a gift from Mathew Bennett (Addgene plasmid # 60733; http://n2t.net/addgene :60733; RRID: Addgene_60733 ). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD-mTagBFP2 plasmid , a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene :34632; RRID: Addgene_34632 ).

    Article Title: Nonequilibrium polysome dynamics promote chromosome segregation and its coupling to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid (Shis and Bennett, 2013), which was a gift from Mathew Bennett (Addgene plasmid # 60733; http://n2t.net/ addgene: 60733; RRID:Addgene_60733). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD- mTagBFP2 plasmid (Subach et al., 2011), a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene: 34632; RRID:Addgene_34632).

    Article Title: DNA/polysome phase separation and cell width confinement couple nucleoid segregation to cell growth in Escherichia coli
    Article Snippet: .. The pET28:mTagBFP2 plasmid variant was derived from the pET28:GFP plasmid , which was a gift from Mathew Bennett (Addgene plasmid # 60733 ; http://n2t.net/addgene:60733 ; RRID:Addgene_60733). .. First, the GFP coding sequence was substituted with the mTagBFP2 coding sequence from the pBAD-mTagBFP2 plasmid , a gift from Vladislav Verkhusha (plasmid # 34632; http://n2t.net/addgene:34632 ; RRID:Addgene_34632).



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    Image Search Results


    a In a traditional continuous cultivation, E. coli is grown with glucose, a carbon source providing a high growth rate, and tasked with the production of a burdensome protein. The important switching cost leads to a highly heterogeneous population that harms population productivity. b Trying to reduce this heterogeneity by applying periodic pulses can lead to mutational escape, resulting in a population that is more homogeneous but less productive. c An alternative way of reducing the switching cost is to reduce the maximum growth rate of the cells, which can be done by lowering the quality of the main carbon source. This enables the creation of a more productive and homogeneous population.

    Journal: bioRxiv

    Article Title: Lowering the switching cost related to the activation of burdensome gene circuits promotes cell population homogeneity and productivity

    doi: 10.1101/2024.10.14.618176

    Figure Lengend Snippet: a In a traditional continuous cultivation, E. coli is grown with glucose, a carbon source providing a high growth rate, and tasked with the production of a burdensome protein. The important switching cost leads to a highly heterogeneous population that harms population productivity. b Trying to reduce this heterogeneity by applying periodic pulses can lead to mutational escape, resulting in a population that is more homogeneous but less productive. c An alternative way of reducing the switching cost is to reduce the maximum growth rate of the cells, which can be done by lowering the quality of the main carbon source. This enables the creation of a more productive and homogeneous population.

    Article Snippet: Experiments performed in this work focused on E. coli BL21 DE3 with a pET28:GFP plasmid expressing EGFP under the control of P T7/lacO (Plasmid #60733) deposited on Addgene by Matthew Bennett.

    Techniques: